HEPES Hemisodium -- CAS No: 103404-87-1
| CAS Registry Number |
103404-87-1 |
| Name |
HEPES hemisodium salt |
| Synonyms |
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) hemisodium salt |
| Molecular Formula |
C8H17.5N2O4S • 0.5Na |
| Molecular Weight |
249.30 |
| Molecular Structure |
  |
| Test |
H1121, High Purity |
H1123, Ultra Pure |
H1125, Ultra BioPure |
| Appearance |
White powder |
White powder |
White crystalline powder |
| Assay (by titration, on dried basis) |
≥ 98.0% |
≥ 99.0% |
≥ 99.0% |
| Solubility (1M solution in H2O) |
-- |
Clear, complete and colorless |
Clear, complete and colorless |
| Identification (by IR) |
Conforms to structure |
Conforms to structure |
Conforms to structure |
| Water (105°C for 3 hours) |
≤ 3.0% |
≤ 3.0% |
≤ 3.0% |
| Water (KF) |
-- |
≤ 2.0% |
≤ 2.0% |
| pH (1% solution in water) |
6.8 – 8.2 |
6.8 – 8.2 |
6.8 – 8.2 |
| pKa (@ 25ºC) |
-- |
7.4 – 7.7 |
7.4 – 7.7 |
| UV Absorption (0.1M @290nm) |
-- |
≤ 0.05 |
≤ 0.05 |
| UV Absorption (0.1M @280nm) |
-- |
≤ 0.05 |
≤ 0.05 |
| UV Absorption (0.1M @260nm) |
-- |
≤ 0.05 |
≤ 0.05 |
| Insoluble Matter |
-- |
≤ 0.1% |
≤ 0.1% |
| Heavy Metals (Lead) |
-- |
≤ 0.0005% |
≤ 0.0005% |
| Biological Impurity Testing |
| Test |
H1121, High Purity |
H1123, Ultra Pure |
H1125, Ultra BioPure |
| Endotoxin (0.1% solution in H2O) |
-- |
-- |
≤ 0.1 EU/mL |
| Bioburden |
-- |
-- |
≤ 100 CFU/g |
| Trace Impurity Testing |
| Test |
H1121, High Purity |
H1123, Ultra Pure |
H1125, Ultra BioPure |
| Iron (Fe) |
-- |
≤ 0.0005% |
≤ 0.0005% |
| Specification Sheet |
| Specification Sheet |
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